[InSCREENeX] HEK293 Freezing Medium A

[InSCREENeX] Products

[InSCREENeX] HEK293 Freezing Medium A

Product Details:

• INS-SU-1025

• Cryopreservation Medium
• Ready-to-use
• 30ml

• Ready-to-use
• Optimized for high viability
• Contains serum

Contents:

• 30ml Freezing Medium ready-to-use

• HEK293 cells (Type-A)

• 70% (v/v) HEK293 Growth Medium A (INS-ME-1041) without Geneticin G418 Sulfat
• 20% (v/v) FBS/FCS
• 10% (v/v) DMSO

• Store the products as follows if not indicated otherwise on the product label. Freezing Medium -18 to -20°C, avoid repeated freeze-thaw cycles. Aliquot if necessary. Please see the product label for the expiry date of your product.

• Aspirate medium.
• Wash with PBS and aspirate PBS.
• Add Trypsin/EDTA (TE) solution to the cells and incubate at room temperature or 37°C for 5-10min, or until the cells detach.
• Examine the cells under a microscope. When the cells start to detach, gently tap the side of the vessel to loosen the remaining cells.
• Resuspend cells in 2% FBS in PBS and transfer to a 15ml conical bottom tube.
• Centrifuge cells at 200*g for 4min.
• Aspirate supernatant and gently resuspend cell pellet in Freezing medium (approx. 1Mio. cells/ml).
• Transfer cell suspension into cryovial(s) and place them into a freezing container (“Mr.Frosty” or similar).
• Place the freezing container at-80°C for 16-24h.
• Transfer cryovials to liquid nitrogen vapor for long-term-storage.